Journal: bioRxiv

Article Title: SARS-CoV-2 infection is associated with intestinal permeability, systemic inflammation, and microbial dysbiosis in hospitalized COVID-19 patients

doi: 10.1101/2023.12.07.570670

Figure Lengend Snippet: Plasma concentrations for I-FABP ( A ), Zonulin ( B ), LBP ( C ), and sCD14 ( D ) measured by ELISA and circulating fatty acids propionic acid ( E ), decanoic acid ( F ), butyric acid ( G ), nonanoic acid ( H ), and isovaleric acid ( I ) measured by LC-MS/MS. Comparisons between groups were performed by Kruskal-Wallis tests, followed by Dunn post-hoc tests if adjusted p values were below 0.05. Pairwise comparisons between each variable were corrected separately for false discovery rate by the Benjamini-Hochberg method and adjusted p values <0.05 were considered significant.

Article Snippet: Gut barrier damage biomarkers, including LBP (cell sciences CKH113), sCD14 (R&D Systems QK383), zonulin (MyBioSource MBS706368), and I-FABP (R&D Systems DFBP20) were measured using commercially available assays according to manufacturer’s guidelines.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Liquid Chromatography with Mass Spectroscopy

Journal: iScience

Article Title: Dietary fiber reduces mortality from secondary sepsis in a murine model of Clostridioides difficile infection

doi: 10.1016/j.isci.2026.115258

Figure Lengend Snippet: Plasma markers of systemic inflammation and kidney injury in CDI mice across diets (A) PCoA of Canberra distances of mean-normalized sepsis/immune marker concentrations. Points are colored by diet, and shapes indicate whether mice were humanely euthanized due to clinical sickness or survived until the experimental endpoint. Vectors show the correlation of each measured factor with PC1 and PC2, with the vector length indicating the relative strength of the correlation. Only statistically significant vectors are shown (multiple regression with Benjamini-Hochberg multiple test correction, p.adj. < 0.05). (B) Plasma concentration of each marker that significantly differed between diets (blood urea nitrogen (BUN), and immune factors sCD14, CXCL1, IL-10, IL-1B, IL-6, and TNF-a) at sacrifice. Pairwise comparisons of concentrations between diets were calculated using Kruskal-Wallis and Dunn’s post hoc tests, with p -value corrections conducted via Benjamini and Hochberg. Boxplot lines (from top to bottom) depict the 75 th , 50 th (median), and 25 th percentiles, with lines extending from the top/bottom of the boxplot indicating the largest/smallest observation within ±1.5∗IQR (inter-quartile range). P-value significance (∗∗∗∗: p < 0.0001, ∗∗∗: p < 0.001, ∗∗: p < 0.01, and ∗: p < 0.05).

Article Snippet: Soluble CD14 (sCD14) levels were quantified using the Quantikine Mouse sCD14 ELISA Kit (Catalog No. MC140, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Marker, Plasmid Preparation, Concentration Assay

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders

doi: 10.1007/s00406-016-0759-5

Figure Lengend Snippet: Twin correlations and ACED estimates based on the best-fitted model according to Akaike’s information criterion (AIC)

Article Snippet: For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN).

Techniques:

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders

doi: 10.1007/s00406-016-0759-5

Figure Lengend Snippet: Results of the co-twin control analysis of the cerebrospinal fluid markers in the disease-discordant twin pairs

Article Snippet: For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN).

Techniques: Control

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders

doi: 10.1007/s00406-016-0759-5

Figure Lengend Snippet: Comparison of sCD14 levels between the twin pairs where the probands are affected with schizophrenia or bipolar disorder and the co-twins are not affected and the line represents each pair ( n = 11)

Article Snippet: For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN).

Techniques: Comparison

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders

doi: 10.1007/s00406-016-0759-5

Figure Lengend Snippet: Co-twin control analysis of sCD14 in cerebrospinal fluid stratified by zygosity, diagnosis in the proband, and antipsychotic medication in the proband

Article Snippet: For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN).

Techniques: Control, Biomarker Discovery

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders

doi: 10.1007/s00406-016-0759-5

Figure Lengend Snippet: Results from the mixed regression analysis between the CSF markers and psychometric scales for psychotic symptoms and personality traits

Article Snippet: For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN).

Techniques:

Journal: European Archives of Psychiatry and Clinical Neuroscience

Article Title: Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders

doi: 10.1007/s00406-016-0759-5

Figure Lengend Snippet: Correlations of the difference scores between the twin pairs ( n = 17) of CSF/serum albumin ratio in relation to the CSF markers sCD14 and YKL-40

Article Snippet: For this reason, we denote MSD-derived Aβ concentrations AβX-38, AβX-40 and AβX-42 and AlzBio3-derived Aβ concentrations Aβ1-42 throughout the manuscript. sCD14 and YKL-40 were determined by Human sCD14 quantikine ELISA kit and Human chitinase-3 quantikine ELISA kit, respectively (R&D systems, Inc, Minneapolis, MN).

Techniques:

Journal: Journal of neurovirology

Article Title: Increased Cell-free Mitochondrial DNA is a Marker of Ongoing Inflammation and Better Neurocognitive Function in Virologically Suppressed HIV-infected Individuals

doi: 10.1007/s13365-016-0497-5

Figure Lengend Snippet: Multivariate associations of clinical variables while adjusting for AIDS status

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) was used to quantify the levels of sCD163 (Trillium Diagnostics, Brewer, ME, USA), sCD14 Quantikine ELISA Human sCD14 R&D Systems, MN, USA and neopterin (BRAHMS Neopterin EIA GmbH, Hennigsdorf, Germany) from blood plasma and CSF, and NFL in CSF (Uman Diagnnostics, Sweden).

Techniques: Clinical Proteomics

Journal:

Article Title: Acute-Phase Concentrations of Lipopolysaccharide (LPS)-Binding Protein Inhibit Innate Immune Cell Activation by Different LPS Chemotypes via Different Mechanisms

doi: 10.1128/IAI.73.1.193-200.2005

Figure Lengend Snippet: hd-LBP inhibits binding of R and S LPS chemotypes to sCD14. Binding of R-type LPS from E. coli F515 (Re-LPS) (A), R-type LPS from S. enterica serovar Typhimurium TV 119 (Ra-LPS) (B), and S-type LPS from E. coli O111:B4 (C) to recombinant human sCD14 and the modulating effects of increasing concentrations of LBP were analyzed by native PhastGel electrophoresis. Following incubation at 37°C for 10 min, samples were subjected to automated native electrophoresis on 20% PhastGels and silver staining by using the PhastSystem apparatus. The positions of uncomplexed sCD14, sCD14-LPS complexes, LBP, and the LPS preparations are indicated. Arrows indicate different sCD14-LPS complexes containing most likely LPS of different chain lengths (C). As a loading control, the native samples were subsequently adjusted to a final concentration of 2% (wt/vol) SDS and additionally analyzed by PhastGel SDS electrophoresis and silver staining (lower panels).

Article Snippet: Briefly, R-type LPS from E. coli F515 or S-type LPS from E. coli O111:B4 at a concentration of 0.025 or 0.113 μg/μl, respectively, was incubated in Dulbecco's phosphate-buffered saline (without magnesium or calcium; Life Technologies/GIBCO BRL, Eggenstein, Germany) in the absence or presence of 0.25 μg of recombinant human sCD14 (Biometec, Greifswald, Germany)/μl at 37°C for 10 min. For analysis of LBP effects, recombinant human LBP (Xoma Corp.) was added at final concentrations of 0.0005, 0.0025, 0.025, 0.125, and 0.25 μg/μl.

Techniques: Binding Assay, Recombinant, Electrophoresis, Incubation, Silver Staining, Control, Concentration Assay